Epub 2016 Feb 27.Golli NE1, Jrad-Lamine A2, Neffati H2, Dkhili H2, Rahali D2, Dallagi Y2, El May MV3, El Fazaa S2.Author information1LMBA, Department of Biology, Faculty of Sciences, University of Tunis El Manar, Tunis, Tunisia.Electronic address: nelgolli@yahoo.fr.2LMBA, Department of Biology, Faculty of Sciences, University of Tunis El Manar, Tunis, Tunisia.3Laboratory of Histology-Embryology and Cell Biology, Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis, Tunisia.AbstractElectronic-cigarettes (e-cigarette), the alternative to classic cigarettes are becoming extremely popular but their safety is not still established.Recent studies have showed cytotoxic effects of the electronic cigarette and its recharge e-liquid, in vitro.The present study was designed to evaluate e-cigarette liquid nephrotoxicity in rats.For this purpose, 32 rats were treated for 28 days as follows: Control group was injected intraperitoneally with NaCl 9 g/l; e-cigarette 0% treated group received an intraperitoneal injection of e-liquid without nicotine diluted in NaCl 9 g/l, e-cigarette treated group, received an intraperitoneal injection of e-liquid containing 0.5 mg of nicotine/kg of body weight/day diluted in NaCl 9 g/l and nicotine-treated group received an intraperitoneal injection of 0.5 mg of nicotine/kg of body weight/day diluted in NaCl 9 g/l.

In nicotine group, creatinine level was increased, whereas urea and acid uric levels were decreased.ecigarette perpignanIn e-liquid-exposed groups, levels of uric acid and mainly urea were lower.e cigarette rancho cordovaInterestingly, after e-liquid exposure, oxidative stress status showed increased total protein and sulfhydril content, whereas superoxide dismutase and catalase activities were decreased.e cigarette potters barHowever, the levels of lipid peroxides were not increased after e-liquid exposure.e cigarette veikalsHistological studies identified excess of cells with reduced and dark nuclei exclusively located in the renal collecting ducts.e cigarette waco tx

Thus, e-liquid seems to alter anti-oxidant defense and to promote minor changes in renal function parameters.e cigarette usb pass throughThis preliminary study raises some flags about possible nephrotoxicity of e-cigarette liquids in rats.As some features observed in rats may not be observed in human smokers, additional studies are needed to further qualify conclusions that might be applicable to actual users of e-cigarettes.Copyright © 2016 Elsevier Inc.All rights reserved.KEYWORDS: Histopathology; Kidney injury; Oxidative stress; Rat; e-liquidPMID: 26925498 DOI: 10.1016/j.yrtph.2016.02.012 [Indexed for MEDLINE] MeSH termsAcute Kidney Injury/bloodAcute Kidney Injury/chemically induced*Acute Kidney Injury/pathologyAcute Kidney Injury/physiopathologyAnimalsBiomarkers/bloodCatalase/metabolismCreatinine/bloodElectronic Cigarettes/adverse effects*Injections, IntraperitonealKidney/drug effects*Kidney/metabolismKidney/pathologyKidney/physiopathologyLipid Peroxidation/drug effectsMaleNicotine/administration & dosageNicotine/toxicity*Nicotinic Agonists/administration & dosageNicotinic Agonists/toxicity*Oxidative Stress/drug effectsRats, WistarRisk AssessmentSmoking Cessation/methods*Species SpecificitySuperoxide Dismutase/metabolismTime FactorsToxicity Tests/methodsUrea/bloodUric Acid/bloodSubstancesBiomarkersNicotinic AgonistsUric AcidNicotineUreaCreatinineCatalaseSuperoxide DismutaseFull Text SourcesElsevier ScienceOvid Technologies, Inc.MedicalE-Cigarettes - MedlinePlus Health InformationQuitting Smoking - MedlinePlus Health InformationMiscellaneousUREA - Hazardous Substances Data BankNICOTINE - Hazardous Substances Data Bank

Invite your friends to like this PageEpub 2016 Apr 21.El Golli N1, Rahali D1, Jrad-Lamine A1, Dallagi Y1, Jallouli M1, Bdiri Y1, Ba N2, Lebret M3, Rosa JP3, El May M4, El Fazaa S1.Author information1a Department of Biology , LMBA (Laboratoire de Microbiologie et Molécules Actives), Faculty of Sciences, El Manar University , Tunis , Tunisia .2b INSERM US32, Kremlin-Bicêtre Hospital , Bicêtre , France .3c INSERM U1176, Kremlin-Bicêtre Hospital , Bicêtre , France , and.4d Laboratory of Histology-Embryology and Cell Biology , Faculty of Medicine of Tunis, University of Tunis El Manar , Tunis , Tunisia.AbstractElectronic cigarettes (e-cigarettes) are becoming the fashionable alternative to decrease tobacco smoking, although their impact on health has not been fully assessed yet.The present study was designed to compare the impact of e-cigarette refill liquid (e-liquid) without nicotine to e-liquid with nicotine on rat testis.For this purpose, e-liquid with nicotine and e-liquid without nicotine (0.5 mg/kg of body weight) were administered to adult male Wistar rats via the intraperitoneally route during four weeks.

Results showed that e-liquid with or without nicotine leads to diminished sperm density and viability, such as a decrease in testicular lactate dehydrogenase activity and testosterone level.Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) analysis identified a reduction in cytochrome P450 side-chain cleavage (P450 scc) and 17 beta-hydroxysteroid dehydrogenase (17βHSD) mRNA level, two key enzymes of steroidogenesis.Following e-liquid exposure, histopathological examination showed alterations in testis tissue marked by germ cells desquamation, disorganization of the tubular contents of testis and cell deposits in seminiferous tubules.Finally, analysis of oxidative stress status pointed an outbreak of antioxidant enzyme activities such as superoxide dismutase, catalase and gluthatione-S-transferase, as well as an important increase in sulfhydril group content.Taken together, these results indicate that e-liquid per se induces toxicity in Wistar rat testis, similar to e-liquid with nicotine, by disrupting oxidative balance and steroidogenesis.